CO2: Immunopathologies, Inflammatory Rheumatic Diseases and Biomarkers in Neoplastic Processes

Our group arises from the close and long-lasting collaboration between basic and clinical researchers in the field of Immunology and Rheumatology. Several members of the group have a long tradition in the study of B lymphocyte populations and their differentiation into plasma cells (PCs), responsible for the production of antibodies and therefore humoral immunity. The maturation process from B lymphocytes to CPs goes through a series of phases in which the physiological and phenotypic characteristics of the CPs gradually change until reaching final maturity. In this stage, in which the CPs produce high affinity antibodies against a specific antigen, they are directed and selected in final host tissues, such as the bone marrow, in which said cells, although they lose their ability to proliferate, acquire a status long-lived Our group, in addition to studying the characteristics of the different phases of B lymphocytes and PCs in humans, also studies the transcriptional regulation of a transcription factor, called PRDM1, key in this cell differentiation process. The expression of alternative isoforms to PRDM1 has been over-expressed in multiple myeloma, the tumor counterpart of PCs.

On the other hand, the objective of our group is the study of systemic autoimmune diseases, fundamentally but not exclusively, Rheumatoid Arthritis (RA) and Systemic Lupus Erythematosus (SLE). Although the pathophysiological mechanisms of autoimmune diseases are unknown, they present various autoantibodies as markers of the disease. Our group tries to identify new cellular and/or molecular biomarkers that allow us to predict and reduce the peaks of the disease through the development and design of possible new therapies. The populations of B lymphocytes and autoreactive PCs present in these patients, in which, as previously mentioned, the group has extensive experience, seem to be a good starting point for study to try to develop therapies that eliminate or lessen the adverse effects of these diseases.

• B lymphocytes and their differentiation to plasma cells. Study of the phenotypic characteristics and transcriptional factors that determine the differentiation of B lymphocytes to plasma cells in different tissues and degrees of maturation. Transcriptional factors that may be involved in the development and maintenance of lymphomas and/or myelomas.
• Protein machinery involved in exocytosis or cell secretion. We characterize the proteins that may be key in the mechanism of antibody secretion by plasma cells. The proteins that make up the cellular secretory machinery are involved in any cellular secretion process, including everything from exosomes to interleukins.
• B lymphocytes and autoreactive plasma cells. We studied these cell populations in patients with rheumatic autoimmune diseases, mainly Systemic Lupus Erythematosus and Rheumatoid Arthritis. Phenotypic and molecular characterization of these populations.
• Study of exosomes and gene expression as biomarkers in multiple myeloma and other neoplasias.

B lymphocytes Plasma cells Autoimmunity Rheumatology Inflammatory diseases Myeloma Exosomes

• Principal Investigators:
  • Antonio Campos Caro (PI).
• Investigators:
  • Alejandro García Núñez.
  • Almudena Sampalo Láinz.
  • Antonia María Fernández Rodríguez.
  • Antonio Nieto Díaz.
  • Carmen Rodríguez Hernández.
  • Fermín Medina Varo.
  • Francisco Mora López.
  • Gema Jiménez Gómez.
  • Inmaculada Macías Fernández.
  • Jesús L. Gómez Perales.
  • Nicolás Chozas Candanedo.
  • Raquel de La Varga Martínez.

• Instituto Salud Carlos III. 2019 PI19/01064. PI: Antonio Campos Caro. SILICOSIS POR AGLOMERADOS DE CUARZO ARTIFICAIL; BUSQUEDA DE BIOMARCADORES DIAGNÓSTICOS / PREDICTIVOS DE LA ENFERMEDAD, USO DE PET/TC COMO TÉCNICA DE SEGUIMIENTO DE LA ENFERMEDAD Y ENSAYO CLINICO CON PIRFENIDONA.
• Instituto Salud Carlos III. FIS15/01147. PI: Antonio Campos Caro: Diferenciación de las células plasmáticas humanas en la salud y en la enfermedad: análisis de la expresión génica de célula única en Lupus Eritematoso Sistémico y en Artritis Reumatoide.

• Consejería de Salud. Junta de Andalucía. 2018. RED de Ensayos Clínicos en Metabolismo: Diabetes, Obesidad y Dislipemia RIC-0539-2018.
• Consejería de Salud. Proyectos de Investigación PI-0123-2018. Collaboration. Retinopatía diabética: la inflamación como una nueva diana terapéutica. Papel de los exosomas.
• Iniciativa Territorial Integrada (ITI) 2014-2020 para la provincia de Cádiz, por la Consejería de Salud y por el Fondo Europeo de Desarrollo Regional PI-0032-2017. Collaboration. Predicción de Riesgo de Metástasis a Distancia del Carcinoma de Mama Mediante la Integración de Datos Morfológicos, Inmunohistoquímicos y Genéticos, a Través de Tecnología Big Data.
• Consejería de Salud. Proyectos de Investigación PI-0366-2013. PI: Carmen Rodríguez Hernández. Identificación de factores inductores de la maduración y supervivencia de células plasmáticas autorreactivas y determinación de subpoblaciones linfoides B en lupus eritematoso sistémico y artritis reumatoide.

1. Cano-Cano, F; Alcalde-Estevez, E; Gomez-Jaramillo, L; Iturregui, M; Sanchez-Fernandez, EM; Fernandez, JMG; Mellet, CO; Campos-Caro, A; Lopez-Tinoco, C; Aguilar-Diosdado, M; Valverde, AM; Arroba, AI-Anti-Inflammatory (M2) Response Is Induced by a sp(2)-Iminosugar Glycolipid Sulfoxide in Diabetic Retinopathy-FRONTIERS IN IMMUNOLOGY-2021-12---632132-10.3389/fimmu.2021.632132. QJCR2020:Q1-7,561.
2. Lorenzo, PI; Vazquez, EM; Lopez-Noriega, L; Fuente-Martin, E; Mellado-Gil, JM; Franco, JM; Cobo-Vuilleumier, N; Martinez, JAG; Romero-Zerbo, SY; Perez-Cabello, JA; Canalejo, SR; Campos-Caro, A; Lachaud, CC; Barreda, AC; Aguilar-Diosdado, M; Fuentes, EG; Martin-Montalvo, A; Dolado, MA; Martin, F; Rojo-Martinez, G; Pozo, D; Bermudez-Silva, FJ; Comaills, V; Reyes, JC; Gauthier, BR-The metabesity factor HMG20A potentiates astrocyte survival and reactive astrogliosis preserving neuronal integrity-THERANOSTICS-2021-11-146983-7004--10.7150/thno.57237. QJCR2020:D1-11,556.
3. Santisteban-Espejo, A; Martin-Piedra, MA; Campos, A; Moran-Sanchez, J; Cobo, MJ; Pacheco-Serrano, AI; Moral-Munoz, JA-Information and Scientific Impact of Advanced Therapies in the Age of Mass Media: Altmetrics-Based Analysis of Tissue Engineering-JOURNAL OF MEDICAL INTERNET RESEARCH-2021-23-11--e25394-10.2196/25394. QJCR2020:D1-5,428.
4. Santisteban-Espejo, A; Perez-Requena, J; Atienza-Cuevas, L; Moran-Sanchez, J; Fernandez-Valle, MD; Bernal-Florindo, I; Romero-Garcia, R; Garcia-Rojo, M-Prognostic Role of the Expression of Latent-Membrane Protein 1 of Epstein-Barr Virus in Classical Hodgkin Lymphoma-VIRUSES-BASEL-2021-13-12--2523-10.3390/v13122523. QJCR2020:Q1-5,048.
5. Hervas-Corpion, I; Gallardo-Orihuela, A; Catalina-Fernandez, I; Iglesias-Lozano, I; Soto-Torres, O; Geribaldi-Doldan, N; Dominguez-Garcia, S; Luna-Garcia, N; Romero-Garcia, R; Mora-Lopez, F; Iriarte-Gahete, M; Morales, JC; Campos-Caro, A; Castro, C; Gil-Salu, JL; Valor, LM-Potential Diagnostic Value of the Differential Expression of Histone H3 Variants between Low- and High-Grade Gliomas-CANCERS-2021-13-21--5261-10.3390/cancers13215261. QJCR2020:Q1-6,639.
6. Rodriguez-Hernandez, C; Medrano-Espinosa, O; Hernandez-Sanchez, A-Mental health of the Mexican population during the COVID-19 pandemic-GACETA MEDICA DE MEXICO-2021-157-3228-233--10.24875/GMM.20000612. QJCR2020:Q4-0,302.
7. Giraud, BYJ; Hernandez, CR-PHYSICAL EDUCATION IN TIMES OF COVID-19. ASSESSMENTS BASED ON THE USE OF ICT-REVISTA CONRADO-2021-17-7932-40--QJCR2020:0-0.

1. Abad-Molina C, Vilches-Moreno M, Cárdaba-Arranz M, Nieto A, Orduña-Domingo A. Identification of the DRB4*01:03:01:02N null allele with HLA-DRB1*04 in a Spanish donor. HLA 2019;93(6):471-473 IF: 2.558 Q3.
2. de la Varga-Martínez R, Rodríguez-Bayona B, Campos-Caro A, Añez GA, Medina-Varo F, Rodríguez C. Autoreactive B-lymphocytes in SLE and RA patients: Isolationand characterisation using extractable nuclear and citrullinated antigens bound to immunobeads. Eur J Immunol. 2019 Jul;49(7):1107-1116. IF: 4.5 Q2.

1. Arroba AI, Campos-Caro A, Aguilar-Diosdado M, Valverde ÁM. IGF-1, Inflammation and Retinal Degeneration: A Close Network. Front Aging Neurosci. 2018 Jul 5;10:203. IF: 3.633 Q2.
2. Hervás-Corpión I, Guiretti D, Alcaraz-Iborra M, Olivares R, Campos-Caro A, Barco Á, Valor LM. Early alteration of epigenetic-related transcription in Huntington's disease mouse models. Sci Rep. 2018 Jul 2;8(1):9925. IF: 4.525 Q1.
3. Mellado-Gil JM, Fuente-Martín E, Lorenzo PI, Cobo-Vuilleumier N, López-Noriega L, Martín-Montalvo A, GómezIGH, Ceballos-Chávez M, Gómez-Jaramillo L, Campos-Caro A, Romero-Zerbo SY, Rodríguez-Comas J, Servitja JM, Rojo- Martinez G, Hmadcha A, Soria B, Bugliani M, Marchetti P, Bérmudez-Silva FJ, Reyes JC, Aguilar-Diosdado M, Gauthier BR. The type 2 diabetes-associated HMG20A gene is mandatory for islet beta cell functional maturity. Cell Death Dis. 2018 Feb 15;9(3):279. IF: 5.638 Q1.

1. Valor LM, Rodríguez-Bayona B, Ramos-Amaya AB, Brieva JA, Campos-Caro A. The transcriptional profiling of human in vivo-generated plasma cells identifies selective imbalances in monoclonal gammopathies. PLoS One. 2017 Aug 17;12(8):e0183264. IF: 2.806 Q1.

1. Gómez-Jaramillo L, Romero-García R, Jiménez-Gómez G, Riegle L, Ramos-Amaya AB, Brieva JA, Kelly-Worden M, Campos-Caro A. VAMP2 is implicated in the secretion of antibodies by human plasma cells and can be replaced by other synaptobrevins. Cell Mol Immunol. 2016 Sep 12. PMID: 27616736 IF: 5.897 Q1.

1. Ramos-Amaya A, Rodríguez-Bayona B, López-Blanco R, Andújar E, Pérez-Alegre M, Campos-Caro A, Brieva JA. Survival of human circulating antigen-induced plasma cells is supported by plasma cell-niche cytokines and T follicular helper lymphocytes. J Immunol. 2015;194(3):1031-8. IF: 4.985 Q1.

1. Gómez-Jaramillo L, Delgado-Pérez L, Reales E, Mora-López F, Mateos RM, García-Poley A, Brieva JA, Campos-Caro A. Syntaxin-4 is implicated in the secretion of antibodies by human plasma cells. J Leukoc Biol. 2014 ;95(2):305-12. PMID: 24146186 IF: 4.57 Q1.