The main objective of the INiBICA Genomics Unit is to provide research staff with state-of-the-art equipment, thus promoting the development of outstanding translational biomedical research.

Provides scientific and technical advice for the development of new analysis methods for RNA expression, DNA variability and the characterization of gene function. The Unit offers the necessary tools for all phases of genomic analysis, ranging from sample preparation to analysis and delivery of data to researchers.

EQUIPMENT
  • Research Unit of the Puerta del Mar University Hospital:
    • Sequencer NGS MiSeq (Illumina).
    • Sequencer MinION MK1C (Oxford Nanopore Technologies).
    • Sequencer SeqStudio Genetic Analyzer System (Applied Byosystems).
    • Station qPCR QuantStudio 12k Flex (Applied Byosystems).
    • Accufill automated station for OpenArray (Applied Byosystems).
    • QSep100 automated capillary electrophoresis station (Bioptic Inc.).
    • Blue-Ray gradient thermal cycler (Biotech).
    • Automated fragment sorter Pippin Prep (Age science).
    • Thermal cycler GENEAMP PCR System 2700 (Applied Byosystems).
    • ROTOR-GENE 6000 real-time quantitative PCR (Corbett).
    • Quantitative real-time PCR CFX Connect (Biorad).
    • Molecular imager, FX (BIORAD).
    • Electrophoresis PowerPac Basic (Biorad).
    • Electrophoresis cuvette SEQ-3341 (SCIE-PLAS).
    • Electrophoresis cuvette DNA SUBCELL (BIORAD).
    • GelDoc Go gel documenter (BioRad).
    • Thermomixer C thermal stirrer (Eppendorf).
    • BD Rhapsody Single-Cell Analysis System (BDBiosciences).
  • SC-IBM:
    • CFX Connect Biorad real-time quantitative PCR.
    • Digital PCR (QX200 Digital PCR Reader. Droplet Generator. Plate Sealer. C1000 Deep).
    • Biorad final time icycler thermal cycler.
    • Vertical electrophoresis cuvette Mini-Protean Biorad Tetra System.
    • Horizontal electrophoresis cuvette, large and small.
    • Thermo shaker TS-100C Biosan.
    • Power Hoefer EPS2A200.
    • Power PowerPac Basic Biorad.
    • Biorad Mini Gel-Caster.
APPLICATIONS
  • Obtaining nucleic acids from different biological samples.
  • Conventional PCR, amplification by simple, multiple or nested PCR.
  • Realtime PCR.
  • Absolute or relative quantification studies of gene expression.
  • Allelic discrimination studies using TaqMan probes.
  • Sanger sequencing of PCR products and plasmids.
  • Metagenomics.
  • Panel sequencing.
  • Microbial whole genome sequencing.
  • Fragment analysis.
  • Single cell labeling and capture.
  • Technical advice and joint development of projects.
SERVICES
  • Extraction of nucleic acids.
  • Purification of nucleic acids.
  • Quantification of nucleic acids by spectrophotometry and fluorimetry.
  • Analysis of quality, purity and integrity of nucleic acids.
  • Reading of results by capillary electrophoresis and in agarose gels.
  • Preparation of libraries for NGS sequencing and long fragments.
  • Quality control of libraries (capillary electrophoresis and fluorimetry).
  • NGS sequencing and long fragment.
  • Sanger sequencing and fragment analysis.
  • qPCR projects and OpenArray.
  • Use (without a technician) of equipment (only for internal users duly trained in the use of the equipment).
  • Use (with a technician) according to the needs of the researcher.
CONTACT PERSONS