Histology Units and Imaging Services: Microscopy

Histology Units and Imaging Services: Microscopy

The Histology and Microscopy Unit serves researchers from the different INiBICA's Research Groups, as well as public and private institutions that may require it. It provides scientific-technical support to Research Groups interested in using microscopy techniques in order to analyse biological samples and immuno-detect antigens in cells and tissues. The service covers works at a pre- and post-processing level both in cell and tissue samples, and can be adapted to the techniques specifically required by each Research Group.

EQUIPMENT

U.I.H.U.P.M

    • Microtome: HM325 model (Thermo scientific)
    • Cryotome: Cryotome-E620 (Thermo shadon)
    • Cytospin: CytoSpin 4 (Thermo Shandon)
    • Optical microscopy:
      • Leica Galen III (LEICA)
      • Inverted microscope: Axiovert 40 (Zeiss)
      • Inverted microscope: DIAPHOT (NIKON)
    • Fluorescence microscopy:
      • CKX-RFA (OLYMPUS)
      • Inverted microscope: CKX41 (OLYMPUS)
    • Gas extraction cabinet: Asem en 14175-1-2-3 (ARREDI TECNICI CABARIN)

SC-IBM

    • Microscope: Olympus BX40 with digital camera DP73.
    • Microscope: Leica IMC S40.
    • Confocal microscope: OLYMPUS FLUOVIEW FV1000.
    • Confocal microscope: LEICA TCS-SL - Base microscope: DMIRE2 model.
    • Multiphoton microscopy: Olympus BX61WI with MAI TAI laser.
    • Laser Dissection Microscope: Olympus IX81 mmi Cellcut.
    • Cryostat: Microm HM 525 Thermo..
APPLICATIONS
  • Structural study of normal and pathological cells and tissues.
  • Morphology and structure of microorganisms.
  • Immunocytochemical and histochemical characterization of different cell types.
  • Morphological verification of experimental therapeutic treatments.
  • Structural study of cellular suspensions and isolated organelles.
  • Control of materials through surface observation.
SERVICES
  • Use (without technician) of equipment (only for internal users properly trained in the use of the equipment).
  • Use (with a technician) according to the needs of the researcher.
  • Preparation of the tissues for cutting. Processing for conventional microtomy: postfixation, paraffin inclusion, microtome cutting techniques. Processing for cryostat: postfixation, cryoprotection, freezing and cutting in cryostat.
  • Immunohistochemical staining of tissue sections, and immunocytochemical staining of cell preparations, for conventional and fluorescence microscopy. Protocol setting for the different techniques.
  • Microscopy image photography. Quantification of expression.
  • Advice and support in experimental design, sample preparation methods, data interpretation and presentation.
  • Development / implementation of new applications and image analysis tools that respond to user's needs.
  • Monitoring and users training in the handling of the Unit's equipment.

► BOOKING

► U.I.HUPM RATES

► SC-IBM RATES

CONTACT PERSON
  • SC-IBM: dg.recursoscientificos@uca.es
  • U.I.HUPM: gema.jimenez.g.sspa@juntadeandalucia.es