Histology Units and Imaging Services: Microscopy
The Histology and Microscopy Unit serves researchers from the different INiBICA's Research Groups, as well as public and private institutions that may require it. It provides scientific-technical support to Research Groups interested in using microscopy techniques in order to analyse biological samples and immuno-detect antigens in cells and tissues. The service covers works at a pre- and post-processing level both in cell and tissue samples, and can be adapted to the techniques specifically required by each Research Group.
EQUIPMENTU.I.H.U.P.M
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- Microtome: HM325 model (Thermo scientific)
- Cryotome: Cryotome-E620 (Thermo shadon)
- Cytospin: CytoSpin 4 (Thermo Shandon)
- Optical microscopy:
- Leica Galen III (LEICA)
- Inverted microscope: Axiovert 40 (Zeiss)
- Inverted microscope: DIAPHOT (NIKON)
- Fluorescence microscopy:
- CKX-RFA (OLYMPUS)
- Inverted microscope: CKX41 (OLYMPUS)
- Gas extraction cabinet: Asem en 14175-1-2-3 (ARREDI TECNICI CABARIN)
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- Microscope: Olympus BX40 with digital camera DP73.
- Microscope: Leica IMC S40.
- Confocal microscope: OLYMPUS FLUOVIEW FV1000.
- Confocal microscope: LEICA TCS-SL - Base microscope: DMIRE2 model.
- Multiphoton microscopy: Olympus BX61WI with MAI TAI laser.
- Laser Dissection Microscope: Olympus IX81 mmi Cellcut.
- Cryostat: Microm HM 525 Thermo..
- Structural study of normal and pathological cells and tissues.
- Morphology and structure of microorganisms.
- Immunocytochemical and histochemical characterization of different cell types.
- Morphological verification of experimental therapeutic treatments.
- Structural study of cellular suspensions and isolated organelles.
- Control of materials through surface observation.
- Use (without technician) of equipment (only for internal users properly trained in the use of the equipment).
- Use (with a technician) according to the needs of the researcher.
- Preparation of the tissues for cutting. Processing for conventional microtomy: postfixation, paraffin inclusion, microtome cutting techniques. Processing for cryostat: postfixation, cryoprotection, freezing and cutting in cryostat.
- Immunohistochemical staining of tissue sections, and immunocytochemical staining of cell preparations, for conventional and fluorescence microscopy. Protocol setting for the different techniques.
- Microscopy image photography. Quantification of expression.
- Advice and support in experimental design, sample preparation methods, data interpretation and presentation.
- Development / implementation of new applications and image analysis tools that respond to user's needs.
- Monitoring and users training in the handling of the Unit's equipment.
- SC-IBM: dg.recursoscientificos@uca.es
- U.I.HUPM: gema.jimenez.g.sspa@juntadeandalucia.es
