
The Molecular Quantification service of the Research Unit helps to study interactions between macromolecules and immunodetection of biomarkers. They include enzymes, receptor-ligand interactions, low affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars, and small molecules.
- Research Unit of the Puerta del Mar University Hospital:
- Nanodrop OneC microvolume UV-Vis spectrophotometer (Thermo Scientific).
- Sonicator Bioruptor pico (Diagenode).
- Fluorimeter Qubit Flex (Invitrogen).
- Luminex IntelliFlex (Thermo Fisher Scientific).
- Varioskan Lux (Thermo Fisher Scientific).
- Biophotometer, BIOPHOTOMETER (EPPENDORF), vial reader.
- Spectrophotometer, POWER WAVE (BIOTEK), plate reader.
- Luminometer, Sirius (Berthold).
- Chemidoc, CHEMIDOC XRS (BIORAD).
- CHEMIDOC DIGITAL, IMAGING SYSTEM (BIORAD).
- Ultracentrifuge OPTIMA MAX XP (Beckman Culter).
- SORVALL T21 refrigerated centrifuge.
- Centrifugal Refrigerated Multipurpose Sirena (AFI).
- Ultracentrifuge Optima XPN-100 (Beckman Coulter).
- NanoSight (Malvern Panalytical).
- SC-IBM:
- Fluorimeter, Luminometer and Plate Spectrophotometer Synergy MX (Biotek). Microplate reader, with multi-detection capacity (spectrophotometer, fluorimeter and luminometer)..
- Alphalisa PerkinElmer. AlphaLISA assays are homogeneous no-wash immunoassays with high sensitivity and wide dynamic ranges. AlphaLISA assays are versatile and can be used to detect secreted, intracellular, or membrane-bound analytes.
- Bioplex®200 System. Biorad. The Bio-Plex 200 system is a system that offers protein and nucleic acid researchers a reliable multiplex analysis solution that allows analysis of up to 100 biomolecules in a single sample.
- Bioplex pro II wash Station. Biorad. Microplate wash station for magnetic bead-based assays, includes magnetic plate holder, waste bottle, 2 liquid bottles.
- Chemidoc. Touch Imaging System. The Bio-Rad ChemiDoc Touch and software allow us to reduce the time it takes to analyze the proteins in our samples from a multi-day study to just a few hours. The ChemiDoc tactile imaging system is for gel imaging and Western blot.
- Quantification of DNA, RNA and proteins.
- Detection for Gene Expression Assays (GFP).
- Enzymology.
- Colorimetry.
- Enzyme characterization.
- Biomarkers quantification.
- Microbial quantification.
- ELISA assays.
- LUCIFERASA Activity Assays.
- Visualization of radioactivity in tissue sections, electrophoresis, and thin-layer plates.
- Apoptosis research.
- Simultaneous detection and quantification of multiple proteins (cytokines, chemokines and growth factors, among others) or expressed mRNA.
- Reading of Absorbance, Luminescence and fluorescence, Time Resolved Florescence (TRF) and AlphaScreen. The combination of the different reading methods offers a large number of possibilities in a single device.
- Use (without a technician) of equipment (only for internal users duly trained in the use of the equipment).
- Use (with technician) according to the researcher's need.
- Advice and support in experimental design, sample preparation methods, interpretation and presentation of data.
- Develop and/or implement new applications and tools that respond to user needs.
- Supervision and training of users in the management of the Unit's equipment.
- SC-IBM: dg.recursoscientificos@uca.es.
- Research Unit of the Puerta del Mar University Hospital: gema.jimenez.g.sspa@juntadeandalucia.es.