Molecular and Biochemical Quantitation Units

Molecular and Biochemical Quantitation Units

The Molecular and Biochemical Quantitation Units help to study interactions between macromolecules and biomarker immuno-detection. They include enzymes, receptor-ligand interactions, low-affinity interactions, second messengers, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

EQUIPMENT

U.I.H.U.P.M

    • Biophotometer: BIOPHOTOMETER (EPPENDORF), vial reader
    • Spectrophotometer: POWER WAVE (BIOTEK), plate reader
    • Luminometer: Sirius (Berthold)
    • Chemidoc, CHEMIDOC XRS (BIORAD)
    • CHEMIDOC DIGITAL, IMAGING SYSTEM (BIORAD)
    • Ultracentrifuge OPTIMA MAX XP (Beckman Culter)
    • Refrigerated centrifuge: SORVALL T21
    • Refrigerated centrifuge: SORVALL T21

SC-IBM

    • Fluorometer, luminometer and plate spectrophotometer: Synergy MX (Biotek). Microplate reader with multi-detection capability (spectrophotometer, fluorometer and luminometer).
    • AAlphalisa PerkinElmer. AlphaLISA assays are homogeneous, washless immunoassays with high sensitivity and wide dynamic ranges. AlphaLISA assays are versatile and can be used to detect secreted, intracellular or membrane-bound analytes.
    • Bioplex®200 System.Biorad. The Bio-Plex 200 System is a system that offers protein and nucleic acid researchers a reliable multiplex analysis solution that allows to analyse up to 100 biomolecules in a single sample.
    • Bioplex pro II wash Station. Biorad. Microplate wash station for magnetic bead-based tests, includes magnetic plate holder, waste bottle, 2 liquid bottles.
    • Chemidoc.Touch Imaging System. Bio-Rad ChemiDoc Touch and its software allow us to reduce the time it takes to analyse proteins in our samples from several days to just a few hours. The ChemiDoc Touch imaging system is intended for gel and Western blot images.
APPLICATIONS
  • Quantitation of DNA, RNA and proteins.
  • Detection for gene expression tests (GFP).
  • Enzymology.
  • Colorimetry.
  • Enzymatic characterization.
  • Biomarker quantitation.
  • Microbial quantitation.
  • ELISA tests.
  • LUCIFERASE activity tests.
  • Visualization of radioactivity in tissue sections, electrophoresis and thin-layer plates.
  • Apoptosis research.
SERVICES
  • Use (without technician) of equipment (only for internal users properly trained in the use of the equipment).
  • Use (with a technician) according to the needs of the researcher.
  • Advice and support in experimental design, sample preparation methods, data interpretation and presentation.
  • Development / implementation of new applications and tools that respond to user's needs.
  • Monitoring and users training in the handling of the Unit's equipment.

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► U.I.HUPM RATES

► SC-IBM RATES

CONTACT PERSON

  • SC-IBM: dg.recursoscientificos@uca.es
  • U.I.HUPM: gema.jimenez.g.sspa@juntadeandalucia.es