The Molecular Quantification service of the Research Unit helps to study interactions between macromolecules and immunodetection of biomarkers. They include enzymes, receptor-ligand interactions, low affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars, and small molecules.

EQUIPMENT
  • Research Unit of the Puerta del Mar University Hospital:
    • Nanodrop OneC microvolume UV-Vis spectrophotometer (Thermo Scientific).
    • Sonicador Bioruptor pico (Diagenode).
    • Fluorimeter Qubit Flex (Invitrogen).
    • Luminex IntelliFlex (Thermo Fisher Scientific).
    • Varioskan Lux (Thermo Fisher Scientific).
    • Biophotometer, BIOPHOTOMETER (EPPENDORF), vial reader.
    • Spectrophotometer, POWER WAVE (BIOTEK), plate reader.
    • Luminometer, Sirius (Berthold).
    • Chemidoc, CHEMIDOC XRS (BIORAD).
    • CHEMIDOC DIGITAL, IMAGING SYSTEM (BIORAD).
    • Ultracentrifuge OPTIMA MAX XP (Beckman Culter).
    • SORVALL T21 refrigerated centrifuge.
    • Centrifugal Refrigerated Multipurpose Sirena (AFI).
    • Ultracentrifuge Optima XPN-100 (Beckman Coulter).
    • NanoSight (Malvern Panalytical).
  • SC-IBM:
    • Fluorimeter, Luminometer and Plate Spectrophotometer Synergy MX (Biotek). Microplate reader, with multi-detection capacity (spectrophotometer, fluorimeter and luminometer)..
    • Alphalisa PerkinElmer. AlphaLISA assays are homogeneous no-wash immunoassays with high sensitivity and wide dynamic ranges. AlphaLISA assays are versatile and can be used to detect secreted, intracellular, or membrane-bound analytes.
    • Bioplex®200 System. Biorad. The Bio-Plex 200 system is a system that offers protein and nucleic acid researchers a reliable multiplex analysis solution that allows analysis of up to 100 biomolecules in a single sample.
    • Bioplex pro II wash Station. Biorad. Microplate wash station for magnetic bead-based assays, includes magnetic plate holder, waste bottle, 2 liquid bottles.
    • Chemidoc. Touch Imaging System. The Bio-Rad ChemiDoc Touch and software allow us to reduce the time it takes to analyze the proteins in our samples from a multi-day study to just a few hours. The ChemiDoc tactile imaging system is for gel imaging and Western blot.
APPLICATIONS
  • Quantification of DNA, RNA and proteins.
  • Detection for Gene Expression Assays (GFP).
  • Enzymology.
  • Colorimetry.
  • Enzyme characterization.
  • Biomarkers quantification.
  • Microbial quantification.
  • ELISA assays.
  • LUCIFERASA Activity Assays.
  • Visualization of radioactivity in tissue sections, electrophoresis, and thin-layer plates.
  • Apoptosis research.
  • Simultaneous detection and quantification of multiple proteins (cytokines, chemokines and growth factors, among others) or expressed mRNA.
  • Reading of Absorbance, Luminescence and fluorescence, Time Resolved Florescence (TRF) and AlphaScreen. The combination of the different reading methods offers a large number of possibilities in a single device.
SERVICES
  • Use (without a technician) of equipment (only for internal users duly trained in the use of the equipment).
  • Use (with technician) according to the researcher's need.
  • Advice and support in experimental design, sample preparation methods, interpretation and presentation of data.
  • Develop and/or implement new applications and tools that respond to user needs.
  • Supervision and training of users in the management of the Unit's equipment.
CONTACT PERSONS