Proteomics and Protein Biochemistry Unit
The Proteomics and Protein Biochemistry Unit belonging to the SCIB of the University of Cadiz provides researchers with techniques intended to study and characterise the set of proteins expressed from a genome (proteome): identification and quantitation of proteins, as well as determination of their location, modifications, interactions, activities and functions.
The services offered by the Proteomics Unit include the separation of proteins by one- and two-dimensional electrophoresis, separation of proteins/peptides by liquid chromatography, protein analysis by MALDI-TOF/TOF mass spectrometry and subsequent identification by MS/MS peptide fingerprinting and database search.EQUIPMENT
- Maldi TOF-TOF Autoflex 200 Bruker.
- FPLC Biologic Duo Flow.
- Q-TOF Impact-II Bruker.
- Nano-HPLC Dionex Ultimate 3000 Thermo.
- Micro-HPLC Dionex Ultimate 3000 Thermo.
- Robot Tecan EVO 75 + STANDARD SW.
- SpeedVac DyNA Vap.
- Protean IIxiCall.
- Criterion Biorad.
- Degassing sonicator Fisherbrand FB15052.
- Nitrogen generator ZEFIRO LC / MS.
Possible applications of proteomic techniques cover a wide spectrum of scientific areas, such as:
- Biochemistry, molecular biology and biotechnology: Structural characterization of proteins or protein complexes, measurement of molecular mass and determination of amino acid sequences, determination of regions of interaction between proteins, identification of proteins from gel or solution samples and with different degrees of complexity, identification and characterization of post-translational modifications, absolute and relative quantification of proteins.
- Human healthcare: Search for protein biomarkers related to diseases, search for therapeutic targets.
- Environmental sciences: Identification of protein alterations produced by stress situations, identification of toxicity or contamination markers.
- Separation, purification and characterization of proteins and peptides.
- Identification of proteins present in databases.
- Identification of proteins not present in databases, by means of homology.
- Studies of changes in protein expression.
- Protein characterization: post-translational modifications, mutations, etc.
- SC-IBM: email@example.com