The Histology and Microscopy unit offers a service to researchers from the different INIBICA groups, and public and private institutions that may need it. It provides scientific-technical support to research groups interested in using microscopy techniques for the analysis of biological samples and the immunodetection of antigens in cells and tissues. The coverage of the service offered works at the pre- and post-processing level, in samples of both cells and tissues, and is adapted to the techniques specifically required by each research group.

  • Research Unit of the Puerta del Mar University Hospital:
    • Tabletop Fluorescence Chamber ZOE (BioRad).
    • Microtome model HM325 (Thermo scientific).
    • Cryotome, Cryotome-E620 (Thermo shadon).
    • Cytospin, CytoSpin 4 (Thermo Shandon).
    • Optical microscopy:
      • - Leica Galen III (LEICA).
      • - Inverted microscope, Axiovert 40 (Zeiss).
      • - Inverted microscope, DIAPHOT (NIKON).
    • Fluorescence microscopy:
      • - CKX-RFA (OLYMPUS).
      • - Inverted microscope, CKX41 (OLYMPUS).
    • Asem gas extraction cabin at 14175-1-2-3 (ARREDI TECNICI CABARIN).
  • SC-IBM:
    • Olympus BX40 microscope digitalization camera DP73.
    • Leica IMC S40 microscope.
    • OLYMPUS FLUOVIEW FV1000 confocal.
    • LEICA TCS-SL base microscope model DMIRE2 confocal.
    • Olympus BX61WI multiphoton laser MAI TAI.
    • Microdisector Laser Olympus IX81 mmi Cellcut.
    • Cryostat Microm HM 525 Thermo.
  • Structural study of normal and pathological cells and tissues.
  • Morphology and structure of microorganisms.
  • Immunocytochemical and histochemical characterization of different cell types.
  • Morphological verification of experimental therapeutic treatments.
  • Structural study of cell suspensions and isolated organelles.
  • Control of materials by observing surfaces.
  • Use (without a technician) of equipment (only for internal users duly trained in the use of the equipment).
  • Use (with technician) according to the researcher's need.
  • Preparation of tissues for cutting. Processing for conventional microtomy: post-fixation, paraffin embedding, microtome cutting techniques. Processing for cryostat: post-fixation, cryoprotection, freezing in medium and cryostat sectioning.
  • Processing for cryostat: post-fixation, cryoprotection, freezing in medium and cryostat sectioning.
  • Immunohistochemical staining on tissue sections, and immunocytochemistry on cell preparations, for conventional and fluorescence microscopy. Development of protocols for the different techniques.
  • Microscopy image photography. Expression quantification.
  • Advice and support in experimental design, sample preparation methods, interpretation and presentation of data.
  • Develop and/or implement new image analysis applications and tools that respond to user needs.
  • Supervision and training of users in the management of the Unit's equipment.