Biochemistry and Molecular Biology. Biotechnological Applications

• Jorge Bolívar Pérez:
• E-mail: jorge.bolivar@uca.es.
• Research ID: G-4322-2015.

• Characterization of Human Autoantigens (Functional Characterization of NOA36/ZNF330 Human Autoantigen).
• Rheumatic diseases.
• Genetic modification of E. coli to optimize biotransformation processes of various compounds through metabolic engineering.
• Collaboration with the group of professor María Domínguez Castellano from the Institute of Neurosciences-UMH. Study of cancer using Drosophila as a model organism.

Autoantigens ZNF330 Autoimmunity E. coli Recombinant proteins Cancer Drosophila

• Principal Investigators:
  • Jorge Bolívar Pérez (PI).
• Investigators:
  • Antonio Valle Gallardo (Doctor Researcher).
• Predoctoral Researchers:
  • Raúl Menor Almagro (Rheumatology Specialist Physician - Predoctoral Researcher).
  • Alejandra Sánchez-Briñas Otte (Pre-doctoral Student).

• Ministerio de Economía y Competitividad. Plan Nacional I+D+i - Retos de la Sociedad CTM2016-79089 Collaboration. Revalorización del Biogás del Vertedero mediante un sistema biológico integrado.
• Ministerio de Economía y Competitividad. Plan Nacional I+D+i - Retos de la Sociedad BFU2015-64239-R Collaboration. Crecimiento y Cáncer: de los mecanismos a las aplicaciones terapéuticas.

• Iniciativa Territorial Integrada (ITI) 2014-2020 para la provincia de Cádiz, por la Consejería de Salud y por el Fondo Europeo de Desarrollo Regional PI-0009-2017 Collaboration. Biomarcadores de Respuesta Terapéutica en el Trastorno Bipolar.

1. Authors: Cobo-Ibáñez T, Urruticoechea-Arana A, Rúa-Figueroa I, Martín-Martínez MA, Ovalles-Bonilla JG, Galindo M, Calvo-Alén J, Olivé A, Fernández-Nebro A, Menor-Almagro R, Tomero E, Horcada L, Uriarte-Itzazelaia E, Martínez-Taboada VM, Andreu JL, Boteanu A, Narváez J, Bohorquez C, Montilla C, Santos G, Hernández-Cruz B, Vela P, Salgado E, Freire M, Hernández-Beriain JÁ, Díez-Álvarez E, Expósito L, Fernández-Berrizbeitia O, Velloso-Feijoo ML, Ibáñez-Barceló M, Lozano-Rivas N, Bonilla G, Moreno M, Raya E, Quevedo-Vila VE, Vázquez-Rodríguez TR, Ibáñez-Ruan J, Muñoz-Fernández S, Sánchez-Alonso F, Pego-Reigosa JM. Title: Hormonal dependence and cancer in Systemic Lupus Erythematosus. Journal: Arthritis Care Res (Hoboken). Year: 2019. Volume, first and last pages: 37(5), pp.616-633. Impact factor: 4,530. Quartile/Decile in the year of publication: Q2.
2. Authors: Valle A, Cantero D, Bolivar J. Title: Metabolic engineering for the optimization of hydrogen production in Escherichia coli: A review. Journal: Biotechnology Advances. Year: 2019. Volume, first and last pages: 37(5), pp.616-633. Impact factor: 11,452. Quartile/Decile in the year of publication: Q1.
3. Authors: Callejo-López JA, Ramírez M, Bolívar J, Cantero D. Title: Main Variables Affecting a Chemical-Enzymatic Method to Obtain Protein and Amino Acids from Resistant Microalgae. Journal: Journal of Chemistry. Year: 2019. Volume, first and last pages: Article ID 1390463Q3 CHEMISTRY. Impact factor: 1,727. Quartile/Decile in the year of publication: Q3.
4. Authors: Karapetyan L, Valle A, Bolivar J, Trchounian A, Trchounian K. Title: Evidence for Escherichia coli DcuD carrier dependent FOF1-ATPase activity during fermentation of glycerol. Journal: Sci Rep. Year: 2019. Volume, first and last pages: 20; 18(1): 4279. Impact factor: 4,525. Quartile/Decile in the year of publication: Q1.
5. Authors: de la Calle ME, Cabrera G, Cantero D, Valle A, Jorge Bolivar. Title: : A genetically engineered Escherichia coli strain overexpressing the nitroreductase NfsB is capable of producing the herbicide D-DIBOA with 100% molar yield Journal: Microb Cell Fact. Year: 2019. Volume, first and last pages: 20; 18(1):86. Impact factor: 4,402. Quartile/Decile in the year of publication: Q1.
6. Authors: de la Calle ME, Cabrera G, Cantero D, Valle A, Jorge Bolivar. Title: : Overexpression of the nitroreductase NfsB in an E. coli strain as a whole-cell biocatalyst for the production of chlorinated analogues of the natural herbicide DIBOA. Journal: New Biotechnology. Year: 2019. Volume, first and last pages: 50:9-16. Impact factor: 3,739. Quartile/Decile in the year of publication: Q1.

1. Authors: Brito, J.; Valle, A.; Almengló. F.; Ramírez M.; Cantero, D. Title: Progressive change from nitrate to nitrite as the electron acceptor for the oxidation of H2S under feedback control in an anoxic biotrickling filter. Journal: Biochemical Engineering Journal. Year: 2018. Volume, first and last pages: 139:154-161. Impact factor: 3.371. Quartile/Decile in the year of publication: Q1.

1. Authors: Rúa-Figueroa I, de Castro MF, Andreu JL, Sanchez-Piedra C, Martínez-Taboada V, Olivé A, López-Longo J, Rosas J, Galindo M, Calvo-Alén J, Fernández-Nebro A, Alonso F, Rodríguez-Lozano B, García Vadillo JA, Menor R, Narváez FJ, Erausquin C, García-Aparicio Á, Tomero E, Manrique-Arija S, Horcada L, Uriarte E, Gil S, Blanco R, López-González R, Boteanu A, Freire M, Galisteo C, Rodríguez-Gómez M, Díez-Álvarez E, Pego-Reigosa JM; IISGS (Instituto de Investigación Sanitaria Galicia Sur); SJOGRENSER and RELESSER researchers and EAS-SER GROUP. Title: Comorbidities in patients with Primary Sjögren's Syndrome and Systemic Lupus Erythematosus: A comparative registries-based study. Journal: Arthritis Care Res (Hoboken). Year: 2017. Volume, first and last pages: Vol. 69(1):38-45. Impact factor: 2.418. Quartile/Decile in the year of publication: Q1.
2. Authors: Raúl Menor Almagro, Juan Francisco Rodríguez Gutiérrez, María Auxiliadora Martín-Martínez, María José Rodríguez Valls, Concepción Aranda Valera, José Luís de la Iglesia Salgado. Title: Asociación entre títulos de anticuerpos antinucleares y enfermedades del tejido conectivo en una Unidad de Reumatología. Journal: Reumatol Clin. Year: 2017 Volume, first and last pages: Vol (13):150-5. Impact factor: 0.420. Quartile/Decile in the year of publication: Q3.
3. Authors: Valle A, Cabrera G, Cantero D, Bolivar J. Title: : Heterologous expression of the human Phosphoenol Pyruvate Carboxykinase (hPEPCK-M) improves hydrogen and ethanol synthesis in the Escherichia coli dcuD mutant when grown in a glycerol-based medium. Journal: New Biotechnology. Year: 2017. Volume, first and last pages: Vol (35) 1-12. Impact factor: 3,733. Quartile/Decile in the year of publication: Q1.
4. Authors: Vega FM, Gautier V, Fernandez-Ponce C, Extremera MJ, Altelaar AFM, Millan J, Tellez JC, Hernandez-Campos JA, Conejero R, Bolivar J, Pardal R, Garcia-Cózar FJ, Aguado E, Heck A JR, Duran-Ruiz MC. Title: The atheroma plaque secretome stimulates the mobilization of endothelial progenitor cells ex vivo. Journal: J Mol Cell Cardiol. Year: 2017. Volume, first and last pages: vol 105: 12-2. Impact factor: 5,680. Quartile/Decile in the year of publication: Q1.

1. Authors: Tania Rodríguez-Ramos, Yamila Carpio, Jorge Bolívar, Leonardo Gómez, Mario Pablo Estrada, Carlos Pendón. Title: Nitric Oxide Synthase-dependent immune response against Gram negative bacteria in a crustacean, Litopenaeus vannamei. Journal: Fish Shellfish Immunol. Year: 2016. Volume, first and last pages: Vol (50): 50-55. Impact factor:. Quartile/Decile in the year of publication: Q1.
2. Authors: Menor Almagro R, Jurado Roger A, Rodríguez Gutiérrez JF, Cardiel MH, Salaberri Maestrojuan. Title: Association of Ro-52, Ro-60 and La antibodies with clinical and laboratory features in a referral hospital in Jerez, Spain. Journal: Reumatol Clin. Year: 2016. Volume, first and last pages: Vol. 12:256-62. Impact factor: 0.420. Quartile/Decile in the year of publication: Q3.
3. Authors: Galindo-Izquierdo M, Rodriguez-Almaraz E, Pego-Reigosa JM, López-Longo FJ, Calvo-Alén J, Olivé A, Fernández-Nebro A, Martinez-Taboada V, Vela-Casasempere P, Freire M, Narváez FJ, Rosas J, Ibáñez-Barceló M, Uriarte E, Tomero E, Zea A, Horcada L, Torrente V, Castellvi I, Calvet J, Menor-Almagro R, Zamorano MA, Raya E, Díez-Álvarez E, Vázquez-Rodríguez T, García de la Peña P, Movasat A, Andreu JL, Richi P, Marras C, Montilla-Morales C, Hernández-Cruz B, Marenco de la Fuente JL, Gantes M, Úcar E, Alegre-Sancho JJ, Manero J, Ibáñez-Ruán J, Rodríguez-Gómez M, Quevedo V, Hernández-Beriaín J, Silva-Fernández L, Alonso F, Pérez S, Rúa-Figueroa I; RELESSER Group, from Spanish Society of Rheumatology Systemic Autoimmune Diseases Study Group (EASSER). Title: Characterization of Patients With Lupus Nephritis Included in a Large Cohort From the Spanish Society of Rheumatology Registry of Patients With Systemic Lupus Erythematosus (RELESSER). Journal: Medicine (Baltimore). Year: 2016. Volume, first and last pages: e2891. doi: 10.1097/MD.0000000000002891. Impact factor: 0.597. Quartile/Decile in the year of publication: Q2.

1. Authors: Menor Almagro R, Ruiz Tudela M.M., Girón Úbeda J., Cardiel Rios M.H., Pérez Venegas J.J., García Guijo C. Title: Mielitis Mielitis transversa en síndrome de Sjögren y lupus eritematoso sistémico: presentación de 3 casos Journal: Reumatol Clin. Year: 2015. Volume, first and last pages: Vol. 11:41-4. Impact factor: 0.501. Quartile/Decile in the year of publication: Q3.
2. Authors: Ambriz Y, Menor Almagro R, Campos ID, Cardiel MH. Title: Chronic rheumatic diseases have more impact in quality of life than traditional chronic illnesses. Experience from a general hospital in México. Journal: Reumatol Clin. Year: 2015. Volume, first and last pages: Vol. 11:68-72. Impact factor: 0.501 . Quartile/Decile in the year of publication: Q3.
3. Authors: Vallejo DM, Juarez-Carreño S, Bolivar J, Morante J, Dominguez M. Title: A brain circuit that synchronizes growth and maturation revealed through Dilp8 binding to Lgr3. Journal: Science. Year: 2015. Volume, first and last pages: Vol 350(6262) aac676; Impact factor: 3,328.
4. Authors: Valle A, Cabrera G, Cantero, D. Bolivar, J. Title: Identification of enhanced hydrogen and ethanol Escherichia coli producer strains in a glycerol-based medium by screening in single-knock out mutant collections. Year: 2015. Journal: Microbial Cell Factories. Volume, first and last pages: 14(1):93. Impact factor: 3.744. Quartile/Decile in the year of publication: Q1/D1.
5. Authors: Valle A, Cabrera G, Muhamadali, H, Trivedi, D, Ratray, N, Goodacre, R, Cantero, D., Bolivar J. Title: A systematic analysis of TCA Escherichia coli mutants reveals suitable genetic backgrounds for enhanced hydrogen and ethanol production using glycerol as main carbon source. Journal: Biotechnology Journal. Year: 2015. Volume, first and last pages: 10(11): 1750–1761. Impact factor: 3.781. Quartile/Decile in the year of publication: Q1.
6. Authors: Sanchez-De Melo, I, Grassi P, Ochoa F, Bolivar J, García-Cozar, FJ; Durán-Ruiz MC. Title: N_Glycosylation profile analysis of trastuzumab biosimilar candidates by Normal Phase Liquid Chromatography and MALDI-TOF MS approaches. Journal: Journal of Proteomics. Year: 2015. Volume, first and last pages: Vol 127 (Pt B):225-33; DOI:10.1016/j.jprot.2015.04.012. Impact factor: 3.867. Quartile/Decile in the year of publication: Q1.