GENOMICS
The Genomics Unit at INiBICA has as its primary objective to provide research staff with state-of-the-art equipment, thereby fostering the development of outstanding translational biomedical research.
It provides scientific and technical guidance for the development of new analytical methods related to RNA expression, DNA variability, and the characterization of gene function. The Unit offers the necessary tools for all phases of genomic analysis, ranging from sample preparation to data analysis and delivery to researchers.
EQUIPMENT
- U.I.H.U.P.M.:
- MiSeq NGS sequencer (Illumina).
- MinION MK1C Sequencer (Oxford Nanopore Technologies).
- SeqStudio Genetic Analyzer System sequencer (Applied Biosystems).
- QuantStudio 12k Flex qPCR System (Applied Biosystems).
- Accufill Automated Station for OpenArray (Applied Biosystems).
- QSep100 Automated Capillary Electrophoresis Station (Bioptic Inc.).
- Blue-Ray Gradient Thermal Cycler (Biotech).
- Pippin Prep Automated Fragment Selector (Age Science).
- GENEAMP PCR System 2700 Thermal Cycler (Applied Biosystems).
- ROTOR-GENE 6000 (Corbett) Real-Time Quantitative PCR.
- CFX Connect (Bio-Rad) Real-Time Quantitative PCR.
- Molecular Imager, FX (BIORAD).
- PowerPac Basic electrophoresis power supply (Biorad).
- SEQ-3341 Electrophoresis Tank (SCIE-PLAS).
- SUBCELL DNA Electrophoresis Tank (BIORAD).
- GelDoc Go Gel Imager (BioRad).
- Thermomixer C thermal shaker (Eppendorf).
- BD Rhapsody Single-Cell Analysis System (BD Biosciences).
- C 1000 Touch Thermal Cycler (BioRad).
- CFX Connect Biorad Real-Time Quantitative PCR.
- Digital PCR (QX200 Digital PCR Reader, Droplet Generator, Plate Sealer, C1000 Deep)
- Biorad Icycler Thermal Cycler—End Time.
- Biorad Tetra System Mini-Protean Vertical Electrophoresis Tank.
- Large and small horizontal electrophoresis tanks.
- Biosan TS-100C Thermo Shaker.
- Hoefer EPS2A200 fountain.
- Biorad PowerPac Basic Power Supply.
- Biorad Mini Gel-Caster.
APPLICATIONS
- Extraction of nucleic acids from various biological samples.
- Conventional PCR; amplification via simple, multiplex, or nested PCR.
- Real-Time PCR.
- Studies on the absolute or relative quantification of gene expression.
- Studies of allelic discrimination using TaqMan probes.
- Sanger sequencing of PCR products and plasmids.
- Metagenomics.
- Panel sequencing.
- Whole-genome sequencing of microorganisms.
- Analysis of excerpts.
- Single-cell labeling and capture.
- Technical advice and joint development by Projects.
SERVICES
- Nucleic acid extraction.
- Purification of nucleic acids.
- Quantification of nucleic acids by spectrophotometry and fluorimetry.
- Analysis of the quality, purity, and integrity of nucleic acids.
- Interpretation of results from capillary electrophoresis and agarose gel electrophoresis.
- Preparation of libraries for NGS sequencing and long-read sequencing.
- Quality control of libraries (capillary electrophoresis and fluorimetry).
- NGS and long-read sequencing.
- Sanger sequencing and fragment analysis.
- Projects qPCR and OpenArray.
- Use (without a technician) of equipment (only for internal users who have been properly trained in the use of the Team).
- Use (with a technician) as needed by the research subject.
RESERVATIONS
If you have any trouble downloading the file mentioned above, you can access it via the following link.
CONTACT PERSONS
- SC-IBM UCA: dg.recursoscientificos@uca.es
- U.I.H.U.P.M: laura.jaramillo@inibica.eu
- Genomics Specialist: Estefanía Jurado, PhD (estefania.jurado@inibica.eu / genomica@inibica.es)
- Phone: (+34) 956 003 041 / Extension: 403 041