HISTOLOGY AND MICROSCOPY
The Histology and Microscopy Unit provides services to researchers from the various INiBICA groups, as well as to public and private institutions that may require them. It offers scientific and technical support to research groups interested in using microscopy techniques for the analysis of biological samples and the immunodetection of antigens in cells and tissues. The scope of the service covers both pre- and post-processing of cell and tissue samples and is tailored to the specific techniques required by each research group.
EQUIPMENT
- U.I.H.U.P.M.:
- ZOE Benchtop Fluorescence Chamber (BioRad).
- HM325 Microtome (Thermo Scientific).
- Cytospin, CytoSpin 4 (Thermo Shandon).
- Optical Microscopy:
- Leica Galen III (LEICA).
- Inverted Microscope, Axiovert 40 (Zeiss).
- Inverted microscope, DIAPHOT (NIKON).
- Fluorescence microscopy:
- CKX-RFA (OLYMPUS).
- Inverted microscope, CKX41 (OLYMPUS).
- LSM 900 KMAT Axio Observer 7 Microscope.
- Axio Observer 3/5/7 KMAT inverted fluorescence microscope (Zeiss).
- Asem fume hood, model 14175-1-2-3 (ARREDI TECNICI CABARIN).
- Olympus BX40 microscope with DP73 imaging camera.
- Leica IMC S40 Microscope.
- OLYMPUS FLUOVIEW FV1000 Confocal Microscope.
- LEICA TCS-SL Confocal Microscope, Base Model DMIRE2.
- Olympus BX61WI MAI TAI Laser Multiphoton Microscope.
- Olympus IX81 mmi Cellcut Laser Microdissector.
- Thermo Microm HM 525 Cryostat.
APPLICATIONS
- Structural study of normal and pathological cells and tissues.
- Morphology and Structure of Microorganisms.
- Immunocytochemical and histochemical characterization of different cell types.
- Morphological evaluation of experimental therapeutic treatments.
- Structural study of cellular suspensions and isolated organelles.
- Material inspection through surface observation.
SERVICES
- Use (without a technician) of equipment (only for internal users who have been properly trained in the use of the Team).
- Use (with a technician) as needed by the researcher.
- Preparation of tissues for sectioning. Processing for conventional microtomy: post-fixation, paraffin embedding, and microtome sectioning techniques. Processing for cryostat: post-fixation, cryoprotection, freezing in medium, and cryostat sectioning.
- Cryostat processing: post-fixation, cryoprotection, freezing in medium, and cryostat sectioning.
- Performing immunohistochemical staining on tissue sections and immunocytochemical staining on cell preparations for conventional and fluorescence microscopy. Developing protocols for the various techniques.
- Microscopy image. Expression quantification.
- Advice and support on experimental design, sample preparation methods, and data interpretation and presentation.
- Develop and/or implement new image analysis applications and tools that meet user needs.
- Supervision and training of users in the operation of the Unit's equipment.
RESERVATIONS
If you have any trouble downloading the file mentioned above, you can access it via the following link.
CONTACT PERSONS
- SC-IBM UCA: dg.recursoscientificos@uca.es
- U.I.H.U.P.M: laura.jaramillo@inibica.eu