HISTOLOGY AND MICROSCOPY
The Histology and Microscopy Unit provides services to researchers from different INiBICA groups, as well as public and private institutions that may require them. It provides scientific and technical support to research groups interested in using microscopy techniques for the analysis of biological samples and the immunodetection of antigens in cells and tissues. The service covers pre- and post-processing of both cell and tissue samples and is tailored to the specific techniques required by each research group.
EQUIPMENT
- U.I.H.U.P.M:
- ZOE tabletop fluorescence camera (BioRad).
- HM325 microtome (Thermo Scientific).
- Cytospin, CytoSpin 4 (Thermo Shandon).
- Optical Microscopy:
- Leica Galen III (LEICA).
- Inverted microscope, Axiovert 40 (Zeiss).
- Inverted microscope, DIAPHOT (NIKON).
- Fluorescence microscopy:
- CKX-RFA (OLYMPUS).
- Inverted microscope, CKX41 (OLYMPUS).
- LSM 900 KMAT Axio Observer 7 microscope.
- Axio Observer 3/5/7 KMAT inverted fluorescence microscope (Zeiss).
- Asem gas extraction cabinet in 14175-1-2-3 (ARREDI TECNICI CABARIN).
- Olympus BX40 microscope with DP73 camera digitization.
- Leica IMC S40 microscope.
- OLYMPUS FLUOVIEW FV1000 confocal microscope.
- LEICA TCS-SL Confocal Microscope, base model DMIRE2.
- Olympus BX61WI MAI TAI laser multiphoton microscope.
- Olympus IX81 mmi Cellcut Laser Microdissector.
- Microm HM 525 Thermo cryostat.
APPLICATIONS
- Structural study of normal and pathological cells and tissues.
- Morphology and structure of microorganisms.
- Immunocytochemical and histochemical characterization of different cell types.
- Morphological verification of experimental therapeutic treatments.
- Structural study of cell suspensions and isolated organelles.
- Material control through surface observation.
SERVICES
- Use (without a technician) of equipment (only for internal users who are properly trained in the use of Team).
- Use (with technician) according to the researcher's needs.
- Preparation of tissues for cutting. Processing for conventional microtomy: post-fixation, paraffin embedding, microtome cutting techniques. Processing for cryostat: post-fixation, cryoprotection, freezing in medium, and cryostat cutting.
- Cryostat processing: post-fixation, cryoprotection, freezing in medium, and cryostat sectioning.
- Performing immunohistochemical staining on tissue sections and immunocytochemistry on cell preparations for conventional and fluorescence microscopy. Fine-tuning protocols for different techniques.
- Microscopy image photograph. Expression quantification.
- Advice and support in experimental design, sample preparation methods, data interpretation, and presentation.
- Develop and/or implement new image analysis applications and tools that meet user needs.
- Supervision and training of users in the handling of the Unit's equipment.
RESERVATIONS
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CONTACT PERSONS
- SC-IBM UCA: dg.recursoscientificos@uca.es
- U.I.H.U.P.M: laura.jaramillo@inibica.eu