PROTEOMICS
The Proteomics Unit, part of the Central Biomedical Research Services at the University of Cádiz, offers analytical services to the scientific community at INiBICA and the University of Cádiz, as well as to researchers from other universities, hospitals, and companies.
The Unit has two main lines: (i) a liquid chromatography-mass spectrometry (LC-MS) platform for performing massive proteomic analyses, both qualitative and quantitative; and (ii) a mass spectrometry-based molecular imaging platform (MALDI Imaging) that provides information on the location of proteins, peptides, lipids, and metabolites directly on tissue sections.
As a support service for research activities, the Proteomics Unit provides personalized support for each project, which may include the stages prior to and following analysis: experiment design, Projects review, bioinformatic analysis, and biological interpretation of the data generated.
EQUIPMENT
- Q-TOF mass spectrometer with ion mobility: Tims-TOF Pro (Bruker).
- MALDI-TOF/TOF mass spectrometer: Autoflex Speed (Bruker).
- nanoHPLC: nanoElute (Bruker).
- Sprayer: HTX M3.
- Main programs used for data analysis: combination of search engines (Peaks, X!Tandem, MSGF+, and Comet), Peaks and Spectronaut for quantitative diaPASEF proteomics, Skyline for PRM-PASEF; SCILS for MALDI imaging, MBT Compass Library for MALDI Biotyper.
SERVICES
- Identification and characterization of proteins by nanoHPLC-MS/MS DDA-PASEF and database search.
- Specialized applications:
- Massive protein identification.
- Identification of PTMs.
- Metaproteomics.
- Quantitative proteomics:
- DIA (data-independent acquisition): diaPASEF.
- Label-free.
- Targeted Proteomics: PRM-PASEF.
- Specialized applications:
- Differential expression in two or more sample groups.
- Discovery/validation of biomarkers.
- Characterization of molecular mechanisms.
- Quantification of PTMs.
- MALDI Imaging.
- MALDI Biotyper (identification of strains and species of microorganisms by MALDI-TOF).
- Review of proposals for Projects calls.
- Collaboration in Projects .
- Training in proteomic and bioinformatic tools.
RATES
If you have any problems downloading the above file, you can access it via the following link link.
CONTACT PERSONS
- Dr. Mª Carmen Durán Ruiz: maricarmen.duran@gm.uca.es
POSTAL ADDRESS
Proteomics Unit.
Central Health Sciences Research Services.
General Affairs Building, University of Cádiz.
C/ Doctor Marañón, 3, 3rd floor. 11002, Cádiz, Spain.